W. EH. Marshall

were most degenerated in the spleen, which was heavily infected with tubercle, and were best preserved in the bone-marrow, where the tubercles were scanty. The post-mortem examination was done immediately after death, so the change in the parasites could not be

a post-mortem one.

Monkey E was infected by the 54th day, became very ill with | diarrhoea on the 143rd day, and was chloroformed on the 145th day. It was heavily infected with parasites. Monkey N, inoculated intraperitoneally from monkey H, re- mained quite well, but, owing to Leishman bodies being present in monkey § (vide infra), liver puncture was carried out, and

- doubtful Leishman bodies were seen 35 days later, the 155th day; the monkey died, and the post-mortem examination showed the spleen to be enlarged, and parasites were found in the spleen,

liver, and bone-marrow. ‘There was a small ulcer in the ileum. Monkey P, which died on the 134th day, was heavily infected with parasites.

Of those infected subcutaneously, in the case of monkey R the injection was made into the subcutaneous tissues of the thigh with

a post-mortem splenic emulsion from a case of kala-azar. On the 150th day the spleen was found to be enlarged, and liver puncture showed the presence of Leishman bodies. On the 156th day the peripheral blood was examined, and in the fifth slide three parasites were found inside a large mononuclear cell. This monkey is still alive.

Monkey L received an intravenous and subcutaneous injection ; it was intended to inject intravenously, but only the first portion of the virus entered the vein, the remainder entering the sub- cutaneous tissues of the forearm. Hxamined on the l16lst day the spleen was found to be enlarged, and spleen puncture showed the presence of Leishman parasites. This monkey is also still alive.

The remaining monkey, which was a very young animal, was put in the same cage as monkey N on the day that the latter was inoculated intraperitoneally from monkey HE. It remained in that cage, and on the 112th day it became ill. On the 117th day liver puncture showed the presence of Leishman bodies, and on the 121st day it died, thirty-four days before monkey N. It was very heavily infected, parasites being present in large numbers in the spleen, liver, and bone-marrow.

With regard to the negative cases, monkey H was inoculated with a splenic emulsion from a case of kala-azar, the emulsion ‘being made eight hours after the death of the patient, when no

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